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2020-08-07
Answer: The myeloma cells before fusion have poor viability, or are contaminated by mycoplasma, the survival rate of spl...
2020-08-07
Answer: 1. Do not shake the petri dish within 10 days. The clones in the methylcellulose semi-solid medium may be broken...
2020-08-07
Answer: 1. The serum may not be completely removed, use MediumB to wash the cells. 2. The concentration of PEG is low. T...
2020-08-07
Answer: Please do not move or shake the petri dish casually. After 10 days, the growth of the clone can be observed. The...
2020-08-07
Answer: KG1 cells, in addition, it is possible that the semi-solid medium was shaken during the culture process, and the...
2020-08-07
Answer: The culture medium and the nail fiber are not well mixed. After adding the cells and culture medium to the semi-...
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