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Belden Biology went to Guangzhou Institute of Biology to exchange colony cultivation technology

Issuing time:2020-08-06 14:19

http://www.gibh.cas.cn/xwdt/zhxw/201409/t20140930_4218583.html


Belden Biotech Mao Xin went to Guangzhou Institute of Biology to exchange colony cultivation technology


On September 24th, the Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences held a lecture entitled "Common methods of hematopoietic stem cell research and analysis of common problems in colony analysis experiments". Mao Xin, general manager of Hangzhou Belden Biotechnology Co., Ltd. was invited to give a lecture The lecture was hosted by researcher Wang Jinyong from the Stem Cell Institute of Guangzhou Academy of Biology. A number of researchers, research assistants, and students in the field of hematopoietic stem cell research attended the lecture.


   Mao Xin focused on the Long-Term Culture-Initiating Cell Assay (LTC-IC), colony formation experiment and analysis techniques of hematopoietic stem cells, and solutions to common problems. The LTC-IC experiment is currently a widely used in vitro experimental method for calculating the number of hematopoietic stem cells. The hematopoietic stem cells are co-cultured with specific feeder cells for more than 4 weeks, and the presence of hematopoietic stem cells in the culture starting cells is verified by colony analysis; Stem cell colony formation analysis is mainly used to detect the presence, type and quantity of hematopoietic progenitor cells in the target cell population. From the perspectives of the starting number of hematopoietic stem/progenitor cells from different sources, the observation time of different colonies, the identification criteria of different colonies, the maintenance of the humidity of the colony culture environment, the introduction of potential pollution, etc., he explained that beginners are easy to appear when doing colony culture Problems and solutions.


   The atmosphere of the meeting was active and interactive. Mao Xin gave detailed answers to many questions encountered by participants in the hematopoietic stem cell colony analysis experiment. The lecture will help improve the colony analysis experiment level of scientific researchers in the field of hematopoietic stem cells in the Academy of Biology.


From: GIBH


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