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Q: The hybridoma cells do not fuse well?

Issuing time:2020-08-07 09:21

Answer: 1. The serum may not be completely removed, use MediumB to wash the cells. 2. The concentration of PEG is low. The supernatant should be completely removed before adding PEG and the cell clusters should be broken up. 3. Excessive exposure of cells to PEG leads to cell death

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