Question: Is it possible to grow red colonies only?

There is no combination of cytokines that allows only erythroid lines or only granulocytes to grow. 10 ng/mL IL-3, 50 ng/mL SCF and 3 U/mL EPO can make erythroid CFU-E and BFU-E achieve near-optimal growth. The combination of EPO and SCF can make the red line grow larger than using EPO alone but cannot get the maximum number. At the same time, CFU-GM and CFU-GEMM will also exist, but it will be much less than in the medium supplemented with GM-CSF.

MethoCult? H4330 can be used, but the number and size of CFU-E and BFU-E will be less and smaller than in the medium supplemented with IL-3, SCF and GM-CSF. Note: The serum in the medium will support the growth of small CFU-GM, but the amount is not large.