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革兰氏阴性菌总蛋白提取试剂盒
产品号:SB-004
规格:50次
 
 
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Description
Invent Biotechnologies MinuteTM bacterial totalprotein extraction kit is composed of
optimized denaturing cell lysis buffers and protein extraction filtercartridges with 2.0 ml
collection tubes. The kit is designed to rapidly extract denatured proteins frombacteria.
The kit contains sufficient materials for extraction of total proteins from 100ml
E.coli culture.
Application
MinuteTM bacterial totalprotein extraction kit is designed to rapidly extract total proteins
from cultured bacterial cells for applications such as SDS-PAGE,immunoblottings,
ELISA and other applications. This kit provides the most rapid method forpreparation of
whole bacterial cell extract.
Buffer Formulation: Proprietary
Kit Components
1. 25 ml bacterial lysis buffer A (Part#: SB-004-A)
2. 2.5 ml buffer B (Part#: SB-004-B)
3. 50 protein extraction filter cartridges (Part#: P-001)
4 50 collection tubes with cap (Part#: P-002)
Storage
Store at room temperature.
Important Product Information
The MinuteTM bacterial total protein extraction kit isdesigned to extract total protein
rapidly from bacteria. The use of protease inhibitors is not necessary prior toextraction.
However if downstream application takes significant amounts of time or theprotein
extract will be stored for longer period of time, addition of proteaseinhibitor to extracted
lysate is recommended. For determination of protein concentration BCA kit(Pierce) is
recommended. The protein extraction filter cartridge has the capacity of 500µl. Multiple
filter cartridges may be used if larger amount of cell lysate is desired. Tostudy protein
phosphorylation, phosphatase inhibitors must be added to lysis buffer prior touse.
 
 
 
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