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植物叶绿体分离试剂盒
产品号:CP-011
规格:50次
 
 
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Description Invent Biotechnologies MinuteTM chloroplast isolation kit is composed of optimizedchloroplast isolation buffers and filter cartridges with 2.0 ml collectiontubes. The kit is designed to rapidly isolate intact chloroplasts from freshplant tissues (leaves, seeds and soft stems etc.). Due to the use of filtercartridges with pre-defined pore size and thickness, intact chloroplasts can beisolated from 5-200 mg fresh plant tissues in less than 5 min. Unlike manyother methods that require 1-10+ gram tissues for chloroplast isolation, thiskit can quickly obtain 1 X 106 to 1 X 107 intact chloroplasts (>90% intact) from fresh plantleaves.


Application MinuteTM chloroplastisolation kit is designed to rapidly isolate intact chloroplasts from smallsamples (5-200 mg) of fresh plant tissues for applications such as biochemicalanalysis, SDS-PAGE, immunoblottings, enzyme assays etc. Isolated chloroplastsare ideal starting materials for purification of RNA and DNA for molecularbiology studies.


Buffer Formulation: Proprietary


Kit components 1. 25 ml buffer A 2. 25 ml buffer B 3. 50 proteinextraction filter cartridges 4. 50 collection tubes with cap 5. Plastic rod (4)
Storage: Store buffer A and B at -20oC


Additional Materials Required Table-Top Microcentrifuge 1 X PBS or 1 XTES buffer

 
 
 
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