English   搜索
InventBiotech蛋白提取产品
 
 
  总蛋白提取 首页 > 产品  
 
 
植物组织总蛋白提取试剂盒
产品号:SD-008/SN-009
规格:50次
 
 
暂无相关产品
 
重点推荐产品
 
TeSR™-E8™
StemSpan CC100细胞因子组合, 1 ml
人间充质干细胞扩增试剂盒, 500ml
StemSpan H3000无血清, 无动物成分液体培基,100ml
甲基纤维素培养基MethoCult H4434 Classic,100 mL
 
产品简介
产品资料下载
操作手册下载
实验技巧下载
常见问题解答
操作视频
 

 

Invent Biotechnologies M i n u t eTM total protein extraction kit for plant tissues is composedof optimized protein extraction buffer and protein extraction filter cartridgeswith 2.0 ml collection tubes. The kit is designed to rapidly extract denaturedor native proteins from plant tissues (leaves, seeds, soft stem and rootsetc.). Since protein profiles extracted by denaturing and native cell lysisbuffer are not identical, for a given application one buffer might be superiorto the other. This kit provides both denaturing and native cell lysis buffersfor users to test and select the best one for a specific application. Due tothe use of the protein extraction filter cartridges, total plant solubleproteins can be extracted from 20-200 mg plant tissue in 5-8 min with highprotein yield (2-8 mg/ml)

 

Application
MinuteTM total protein extraction kit for plant tissuesis designed to rapidly extract total proteins from fresh or frozen planttissues for applications such as SDS-PAGE, immunoblottings, ELISA, IP, enzymeassays and other applications. This kit provides a very rapid method forpreparation of soluble protein extract from plant tissues. Extracted proteinscan be use as a good starting material for small scale protein purification incolumn chromatography.


Buffer Formulation: Proprietary


Kit components 1. 25 ml denaturing lysis buffer (SD-008) 2. 25 ml nativelysis buffer (SN-009) 3. 50 protein extraction filter cartridges 4. 50collection tubes with cap 5. Plastic rod (4)


Storage: Store buffer SD-008 at room temperature. Store buffer SN-009 at4oC


Important Product Information The MinuteTM total protein extraction kit for plant tissues is designedto extract total protein rapidly. The use of protease inhibitors is notnecessary prior to extraction. However if downstream application takessignificant amounts of time or the protein extract will be stored for longerperiod of time, addition of protease inhibitors to cell lysis buffer isrecommended. For determination of protein concentration, BCA kit (Pierce) isrecommended. To study protein phosphorylation, phosphatase inhibitors (suchas PhosStop from Roche) should be added to buffer A prior to use.

 
 
 
Copyright © 2010 杭州百通生物技术有限公司 All Rights Reserved.   技术支持:35互联