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Invent Biotechnologies M i n u t eTM total protein extraction kit for plant tissues is composedof optimized protein extraction buffer and protein extraction filter cartridgeswith 2.0 ml collection tubes. The kit is designed to rapidly extract denaturedor native proteins from plant tissues (leaves, seeds, soft stem and rootsetc.). Since protein profiles extracted by denaturing and native cell lysisbuffer are not identical, for a given application one buffer might be superiorto the other. This kit provides both denaturing and native cell lysis buffersfor users to test and select the best one for a specific application. Due tothe use of the protein extraction filter cartridges, total plant solubleproteins can be extracted from 20-200 mg plant tissue in 5-8 min with highprotein yield (2-8 mg/ml)


MinuteTM total protein extraction kit for plant tissuesis designed to rapidly extract total proteins from fresh or frozen planttissues for applications such as SDS-PAGE, immunoblottings, ELISA, IP, enzymeassays and other applications. This kit provides a very rapid method forpreparation of soluble protein extract from plant tissues. Extracted proteinscan be use as a good starting material for small scale protein purification incolumn chromatography.

Buffer Formulation: Proprietary

Kit components 1. 25 ml denaturing lysis buffer (SD-008) 2. 25 ml nativelysis buffer (SN-009) 3. 50 protein extraction filter cartridges 4. 50collection tubes with cap 5. Plastic rod (4)

Storage: Store buffer SD-008 at room temperature. Store buffer SN-009 at4oC

Important Product Information The MinuteTM total protein extraction kit for plant tissues is designedto extract total protein rapidly. The use of protease inhibitors is notnecessary prior to extraction. However if downstream application takessignificant amounts of time or the protein extract will be stored for longerperiod of time, addition of protease inhibitors to cell lysis buffer isrecommended. For determination of protein concentration, BCA kit (Pierce) isrecommended. To study protein phosphorylation, phosphatase inhibitors (suchas PhosStop from Roche) should be added to buffer A prior to use.

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