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胞质/胞核蛋白组分分离试剂盒
产品号:SC-003
规格:50次
 
 
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InventBiotechnologies MinuteTMCytolpasmic andNuclear Extraction Kit is composed of optimized cytoplamic extraction buffer,nuclear protein extraction buffer and protein extraction filter cartridges with2.0 ml collection tubes. The kit is designed to rapidly separate native cytosoland nuclear proteins from cultured mammalian cells or tissues. Due to the useof the protein extraction filter cartridges separation of cytolasmic andnuclear proteins can be accomplished in less than 15 min

 

应用:

The kitis designed to rapidly extract native cytoplasmic and nuclear proteins fromcultured cells or tissues for applications such as SDS-PAGE, immunoblottings,ELISA, IP, protein localization, gel mobility shift assays, 2-D gels and otherapplications. This kit provides the most rapid method currently available forfractionation of native cytoplasmic and nuclear proteins.

 

Kit components

1.  25 ml 胞质提取液  (Part#: SC-003-L)

2.  25 ml 胞核提取液(Part#: SC-003-N)

3.  50个样品处理装置

4.  50个蛋白收集管

 

保存

收到后保存于4℃。

 

重要提示

The useof protease inhibitors is not necessary prior to extraction. However ifdownstream application takes significant amounts of time or the protein extractwill be stored for longer period of time, addition of protease inhibitor toextracted lysate is recommended. The nuclear extraction buffer contains 300 mMsalt, for some applications, dilution or desalting of the extract may beneeded. . For determination of protein concentration, BCA kit (Pierce) isrecommended. The protein extraction filter cartridge has the capacity of 500µl. Multiple filter cartridges may be used if larger amount of cell lysate isdesired. To study protein phosphorylation, phosphatase inhibitors must be addedto lysis buffer prior to use.

 

 
 
 
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